2019-04-11

Outbreak Analysis Using 1928 - Pseudomonas aeruginosa

cgMLST UPGMA-clustering of a Pseudomonas aeruginosa outbreak using the 1928 platform

The tree is created using UPGMA and distances shown are computed based on pairwise allelic differences (hamming distance). Missing genes are not included in the count.

1928's analysis of an outbreak: Pseudomonas aeruginosa

The ambition with 1928’s outbreak analyses is to reproduce results from scientific studies and highlight the quality of the results generated by the 1928 platform.

In order to demonstrate the accuracy of outbreak analysis with the 1928 platform, a phylogenetic tree is created from an outbreak of Pseudomonas aeruginosa. The tree shown in the figure above is generated using the 1928 analysis platform.

In this analysis, 1928 reproduces results from the scientific paper Pseudomonas aeruginosa intensive care unit outbreak: winnowing of transmissions with molecular and genomic typing (pmid: 29229490) by B.J. Parcell, K. Oravcova, M. Pinheiro, M.T.G. Holden, G. Phillips, J.F. Turton, S.H. Gillespie.

The 1928 Pseudomonas aeruginosa cgMLST schema is created from 175 reference genomes giving the scheme 3937 core genes. All genes included in the schema are present in at least 95% of reference genomes.